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Protein concentration was determined using BCA protein assay (Pierce) and an equal amount of each sample was incubated with FY01 at 1 M for 30 min at 37 C to label active cysteine proteases

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That was a big thing for me, which was tracking it because I knew I didnt sleep enough or well, and then tracking it and working on that deep sleep component where the lymphatics of the brain do the washout of all your waste products, which otherwise will inflame your brain

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doi:10.1016/j.neulet.2010.09.040