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Following 24 h of treatment with ALCAR (1 or 10 mM), cells were lysed in RIPA buffer, supplemented with protease and phosphatase inhibitor cocktails (Roche Diagnostics GmbH)

muciniphila degrades mucin and produces a variety of bioactive metabolites, such as polysaccharides, short-chain fatty acids (SCFA), and indole derivatives

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The review concludes with evidence-based recommendations for future investigations, prioritizing target validation studies, standardized clinical trial protocols, and personalized treatment paradigms

4 About the Compounds BPC-157 Compound class: synthetic stable gastric pentadecapeptide