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Tubulin expression was detected on the same PVDF membrane using anti--tubulin specific monoclonal antibody (1 g/ml Sigma-Aldrich, Ont., CA), followed by HRP-conjugated goat anti-mouse IgG (1:5000 (v/v), BioRad, Ont., CA)
These structural modifications reduce affinity for endogenous IGF binding proteins, resulting in altered stability and interaction characteristics in experimental systems compared to native IGF-1
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Efficacy Comparison Compared to other weight loss medications like Orlistat, Liraglutide, and Phentermine-Topiramate, Semaglutide injections often show superior results in terms of the amount of weight loss achieved
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