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2.1.3 50 ns MD simulation of protein-ligand complexes of representative molecules from clusters of human acetylcholinesterase inhibitors and conformational sampling The poses corresponding to the 9 representative molecular structures associated with families C31, C4, C19, C50, C35, C20, C36, C42, and C23 were extracted from the induced-fit calculations

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Bae, S.H., et al.,Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage

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Real-time PCR was performed using an S1000 PCR instrument (Bio-Rad, Hercules, CA, USA) to quantify gene expression