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CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)

This is why devices with high discharge demands often incorporate advanced cooling or airflow systems

doi:10.3390/biomedicines11112925 11

Suspected adverse reactions to vitamin B12 treatments can be reported via the MHRA Yellow Card scheme

Fertil Steril, 2005

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