Van Wagner, L
Homocysteine then follows one of two major pathways: it is either remethylated back into methionine using folate-derived 5-methyltetrahydrofolate (5-MTHF) or enters the transsulfuration pathway to produce cysteine and glutathione, essential for antioxidant defense
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10.1093/ajcn/81.4.736 Am J Clin Nutr
[DOI] [PMC free article] [PubMed] [Google Scholar] 6.Chin B, Ramji M, Farrokhyar F, Bain JR
Western blotting For western blotting experiments, tissues were lysed with Magnalyser beads in radioimmuno-precipitation assay (RIPA) buffer (50 mM Tris-HCl, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1% SDS, 0.5% Nadeoxycholate, 1% NP-40, adjusted to pH 7.27.4) supplemented with PIC (complete Tablets EASYpack, Roche, Basel, Switzerland) and PhosStop (Roche, Basel, Switzerland)