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To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer
5 Additional signs may include a walking pattern that consists of shuffling and short steps without swinging of the arms
Kravchuk N (2012) Hyperhomocysteinemia, cardiovascular disease and type 2 diabetes
This was surprising because the promoters of Wnt genes contain strong CpG islands that usually stay methylation-free (Bird, 2002)
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