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These slices are preserved in liquid nitrogen through vitrification or controlled-rate freezing (slow freezing)

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BPC 157 induced the vasorelaxation via eNOS/nitric oxide singling activation In order to investigate whether BPC 157 induced vasorelaxation via eNOS-nitric oxide singling activation, L-NAME (eNOS inhibitor) and hemoglobin (nitric oxide scavenger) were used in further studies

Before each measurement, the sample was kept in the dark for 15~20 min ( 2 s 1 measuring light (650 nm red LED) to get the initial chlorophyll fluorescence Fo level and then subject to 0.8 s of 4000 mol photons m 2 s 1 saturation pulse to get the maximum chlorophyll fluorescence Fm

et al: Respiration 9:493-8, 2004 2