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516 In the primary cell (sPD cell) derived from patients with sporadic PD, the cytosolic conversion of pyruvate to lactic acid resulted in a significant increase in the nuclear NAD + level and cellular NAD + /NADH ratio

Trial sequential analysis (TSA)

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Following our previously reported protocols with the NanoBiT tethering strategy 11 , the structures of retatrutideGLP-1RG s , retatrutideGIPRG s and retatrutideGCGRG s were determined using the single-particle cryo-EM with overall resolutions of 2.68 , 3.26 and 2.84 , respectively (Fig