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Real-time PCR analysis Total RNA from kidney tissue was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany)

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Take 20 mL of the filtrate, adjust the pH to 12.513.0 using a 4 mol/L potassium hydroxide solution, and place it in a 40C water bath for 60 minutes

The approval of Semaglutide as a weight loss medication opens up new possibilities for achieving sustainable weight loss and improved overall health

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