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Our experiments demonstrate that 10x his-tag does not affect the protein's response to oxidative stress, and that when purifying protein, the length of histidine amino acids should be taken into account

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Conclusions This review of published studies indicates that evidence for the involvement of the -glutamyl cycle and trans-sulphuration pathway in autism spectrum disorders should be further explored with higher quality and lower risk bias studies to in order to ascertain the significance of these pathways with respect to clinical outcomes