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Two weeks of careful observation usually reveals the answer

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As the infection resolves, persistent inflammation can be detrimental to tissue repair, and phagocytes undergo a functional reprogramming toward an anti-inflammatory, pro-resolving phenotype

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Cells were washed with PBS and blocked with 5% BSA for 1 h at RT

A common research approach is to add 2.0 mL of bacteriostatic water to a 10 mg vial, which gives a 5 mg/mL working concentration