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Additionally, ethanol-induced OS is one of the factors involved in the activation of the mammalian target of rapamycin (mTOR), which is essential to premature senescence in cementoblasts and human periodontal ligament cells (Figure 2)

10.1007/s11538-024-01346-5 222

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Cell lysate can be prepared : collect 2 x 10 6 cells by centrifugation at 1,000g for 10 min at 4C