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Primary hepatocytes on Day 2 were stimulated by H 2 O 2 (30% hydrogen peroxide, 7722-84-1) with a concentration gradient of 0, 10, 50, 100, 200 nM for 24 h, or a time gradient of 0, 1, 6, 12, 24 h, or pretreated with 100 ng/ml IGF-1 (Insulin-like growth factor-1, PeproTech, 081701)

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