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Fluorescence was measured after setting up the reaction in the kinetic mode for 60 minutes at 37 C by TECAN kinetic analysis (excitation 360 nM, emission 460 nM, 30-second reading interval) on a Safire II microplate reader (TECAN)

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Bioassay of the serum for thyroid-stimulating activity gave a TSH-type response.* These patients were studied in cooperation with Dr

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