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After washing five times with lysis buffer, the complexes were mixed with lysis buffer containing 290 mM imidazole, and the bound proteins were eluted by boiling in 5 sodium dodecyl sulfate (SDS) loading buffer for 5 min

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Moreover, those trials found statistically significant improvements in sexual-desire scores and reductions in distress related to low desire

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The lower left quadrant includes values within or near the reference range

In most cases, this settles as the body adapts