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While tissue is mounted in the Ussing chamber, a variety of measurements can be collected including transepithelial potential difference and transepithelial electrical resistance (TER) indicating tissue viability and barrier integrity, as well as flux of FITC-dextran, FITC-lipopolysaccharide (LPS), or mannitol to measure tight junction damage and leakage across the mucosa [37]

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Then gradually reintroduce carbohydrate restriction