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Derivatized protein samples were then reduced in 8 M urea, 15 mM dithiothreitol (DTT) at 60 C for 40 min, and then alkylated with 35 mM iodoacetamide (IAM) at room temperature in the dark for 40 min

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Isolation and nature of surface-active substances from Astacus leptodactylus Esch

A lack of methylfolate means this conversion cant happen as it should